Actually, this is related to the software, but no, I would not upgrade. After Mass Hunter 5.01 some checks were added. In older software the autotune only checked that the EM gain was 100,000. In newer software is still checks that but also checks the number of pulse counts. The pulse counts are measured during the gain determination. The error message of ""Insufficient Number of Valid Data Points"" means that
there were not enough valid data points (3) to draw the gain curve. The system needs 100 and 1000 for the point to be
valid for calculating each measurement during the gain curve; If the ponts are greater or less than these values, the point is not considered valid.